The research topics of the Research Institute can be divided into three thematic groups.

The first group is devoted to applied research of African species of animals, which form the basis of aimals bred in Safari Park Dvůr Králové. Applied research of African animal species is focused primarily on issues related to breeding in human care. Special emphasis is also placed on the possible reintroduction of animals from the rescue programs to the areas of their original distribution.

The second group follows up on the above-mentioned issue of reintroductions and addresses practical part related to the reintroduction of animals in general. It including specific examples of native species of the Czech Republic and Europe.

The third group of topics is basic research of animals in zoos. This research in accordance with the mission of modern zoos, aims to expand our knowledge of the biology of species in huan care.